|
KMID : 1101420150470040286
|
|
Korean Journal of Clinical Laboratory Science
2015 Volume.47 No. 4 p.286 ~ p.291
|
|
|
Bacterial Identification and Detection of Equol in Korean Soybean Paste
|
|
Woo Seung-Gyun
Lee So-Yeon
Choi Go-Woon
Hong You-Jin
Lee So-Min
Park Kang-Gyun
Eom Yong-Bin
|
|
|
Abstract
|
|
|
|
Equol has beneficial effects on human health. Fermented soy products contain equol, and many microbes participate in the equol production process. This study investigated fermented Korean soybean paste, doenjang. Thirty seven doenjang samples collected from different manufacturers were examined. Equol was detected in 3 samples (D2, D13, and D19) at the maximum content of 507 ng/100 g by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Fifteen microbial species were isolated and identified by 16S rRNA gene sequence analysis and by matrix assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS). Bacillus spp, Paenibacillus spp, Tetragenococcus spp, Stapylococcus spp, and Clostridium species were the predominant bacteria in equol containing doenjang samples.
|
|
|
KEYWORD
|
|
|
Equol, LC-MS/MS, MALDI-TOF mass spectrometry, 16S rRNA gene sequence
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|